Oh Giovanna Scan
Settings: Trained abdominal radiologists from 2 centers performed a blinded review of CT scans obtained to locally stage proximal colon cancer according to previously defined prognostic groups, including T1/2, T3/4, N+, and extramural venous invasion. CT findings were compared with histopathologic results as a reference standard. Unfavorable pathologic findings included pT3/4, pN+, or extramural venous invasion.
Oh giovanna scan
Results: Of 150 CT scans reviewed, CT failed to identify primary cancer in 18%. Overall accuracy of CT to identify unfavorable pathologic features was 63% with sensitivity, specificity, positive predictive value, and negative predictive value of 63% (95% CI, 54%-71%), 63% (95% CI, 46%-81%), 87% (95% CI, 80%-94%) and 30% (95% CI, 18%-41%). Only cT3/4 (55% vs 45%; p = 0.001) and cN+ (42% vs 58%; p = 0.02) were significantly associated with correct identification of unfavorable features at final pathology. CT scans overstaged and understaged cT in 23.7% and 48.3% and cN in 28.7% and 53.0% of cases.
Conclusions: Accuracy of CT scan for identification of pT3/4, pN+, or extramural venous invasion was insufficient to allow for proper identification of patients at high risk for local recurrence and/or in whom to consider alternative treatment strategies. Locoregional overstaging and understaging resulted in inappropriate treatment strategies in
The demand for rapid, consistent and easy-to-use techniques for detecting and identifying pathogens in various areas, such as clinical diagnosis, the pharmaceutical industry, environmental science and food inspection, is very important. In this study, the reference strains of six food-borne pathogens, namely, Escherichia coli 0157: H7 ATCC 43890, Cronobacter sakazakii ATCC 29004, Salmonella Typhimurium ATCC 43971, Staphylococcus aureus KCCM 40050, Bacillus subtilis ATCC 14579, and Listeria monocytogenes ATCC 19115, were chosen for scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analysis. In our study, the time-consuming sample preparation step for the microbial analysis under SEM was avoided, which makes this detection process notably rapid. Samples were loaded onto a 0.01-µm-thick silver (Ag) foil surface to avoid any charging effect. Two different excitation voltages, 10 kV and 5 kV, were used to determine the elemental information. Information obtained from SEM-EDX can distinguish individual single cells and detect viable and nonviable microorganisms. This work demonstrates that the combination of morphological and elemental information obtained from SEM-EDX analysis with the help of principal component analysis (PCA) enables the rapid identification of single microbial cells without following time-consuming microbiological cultivation methods.
The study of the intimate connection occurring at the interface between cells and titanium implant surfaces is a major challenge for dental materials scientists. Indeed, several imaging techniques have been developed and optimized in the last decades, but an optimal method has not been described yet. The combination of the scanning electron microscopy (SEM) with a focused ion beam (FIB), represents a pioneering and interesting tool to allow the investigation of the relationship occurring at the interface between cells and biomaterials, including titanium. However, major caveats concerning the nature of the biological structures, which are not conductive materials, and the physico-chemical properties of titanium (i.e. color, surface topography), require a fine and accurate preparation of the sample before its imaging. Hence, the aim of the present work is to provide a suitable protocol for cell-titanium sample preparation before imaging by SEM-FIB. The concepts presented in this paper are also transferrable to other fields of biomaterials research.
Citation: Parisi L, Toffoli A, Ghezzi B, Lagonegro P, Trevisi G, Macaluso GM (2022) Preparation of hybrid samples for scanning electron microscopy (SEM) coupled to focused ion beam (FIB) analysis: A new way to study cell adhesion to titanium implant surfaces. PLoS ONE 17(8): e0272486.
Among electron microscopy techniques, scanning electron microscopy (SEM) has long been recognized as the most viable option to image and study the three-dimensional (3D) structure of objects, with no arguably instrument with its breadth of applications. Its function is based on the use of an accelerated electron beam, with a wavelength 100000 shorter than that of light photons, which makes possible to enhance the magnification power of light microscopies (200nm) to 1000-fold (0.2nm). In details, SEM works thorugh an high energy electron beam, which scans across the surface of a conductive specimen, thus inducing the emission of other electrons (secondary), which are collected, processed and converted into 2D images. Over the past years, SEM has been improved with numerous investigation tools. Recently, SEM coupling with an additional column capable to generate a focused ion beam (FIB) for the live milling or cross-sectioning of samples at a glancing defined angle has been proposed [15, 16]. Accordingly, we hypothesized to use SEM-FIB microscopy as a viable tool to study the interaction occurring at the cell-titanium interface . However, the analysis of cellular and biological structures at these high resolution has always been a challenge, because of their intrinsic non-conductive nature.
CLEM allows fully hydrated and potentially live cell specimens to be examined by confocal or multiphoton microscopy, then subsequently prepared for examination for further imaging using electron microscopy. CLEM is applicable for both scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
Ticks are external parasites. They are widely distributed around the world, especially in warm, humid environment. Ticks live by sucking the blood as their nutrition. Because of their blood-ingesting diets and infections caused by pathogens, ticks act as vectors of many serious diseases that affect humans and other animals. Dr. Tong Zhang imaged this sample by taking the advantage of sample auto-fluorescence under different wavelengths with a 10x objective on a Nikon A1R Confocal Microscope at CMIF. There were 423 Z-direction images covering through a 211um thickness. Extended focus function re-built the 3D Z-stack images into a 2D image with 3D information. The microscope resonant scanner boosted the acquisition speed significantly.
Akira wholeheartedly laughed. He motioned for Yusuke to follow him and they separated ways with Makoto, who had made the choice to scan the southern area of Rome while Akira and Yusuke searched the eastern area.
Akira and Yusuke stood by the edge, their eyes scanning the streets below them in search of their target. After a few fruitless moments passed by, Akira turned away and began walking to the other side of the building. Yusuke turned around and followed not too shortly afterwards.
The Phantom Thief took a deep breath to calm himself. Hearing footsteps from above, Akira scanned the surrounding area and found an alleyway just across the street. He glanced up to the rooftop where he fell from. The footsteps soured as though they were coming closer.
Now a massive sphere of gleaming light formed in the Rebel Demon King's hands, though at a slower pace than it's other attacks. Giorno and GER were poised ready to nullify the attack, while Giorno's eyes scanned the surrounding area for the hidden Phantom Thief. He noticed that Akira's companion's bodies had vanished; even that masked cat that had recovered the juvenile's mask was gone.
Everything changed when, after several visits to the ER over six years with unexplainable abdominal pain, each time being told it was stress or irritable bowels, she learned the real cause. After an abdominal scan, Imbesi was diagnosed with a rare form of cancer called a neuroendocrine tumor, or NET.
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